Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLSVKIAGVLTVAAAAMTAKMYATAKGNHLKTHTFPLSKMKGKPPLTIFFISDIHKRLI------------D-QDLLEKARSHAPHLVIIGGDLAEGGVP----SARIEENIKRL-----------------VHFGVPIVFVWGNNDYEVRQHK--LYSIFKAHGVI-TLRN---------ESVPFSYNGHTIAIAGVDDIRMEMD--HY----EEAIKE--LDESQLNILVCHNPEIHEQ----IN---EDDGIDVILSGHTHGGQIRFGKFGPYELGKTGIVKNAAYLISNGYGTTKVPLR-LGAEPETHIVTLCGPE 3T1I Chain:A ((30-311)) -------------------------------------------ENTFKILVATDIHLGFMEKDAVRGNDTFVTLDEILRLAQENEVDFILLGGDLFHENKPSRKTLHTCLELLRKYCMGDRPVQFEILSDQLNISI--PVFSIHGNHDDPTGADALCALDILSCAGFVNHFGRSMSVEKIDISPVLLQKGSTKIALYGLGSIPDERLYRMFVNKKVTMLRPKEDENSWFNLFVIHQNRSKHGSTNFIPEQFLDDFIDLVIWGHEHECKIAPTKN-------EQ---QLFYISQPGSSVVTSLSPGEAVKKHVGLLRIKG--

General information: TITO was launched using: RESULT: Template: 3T1I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 969 20127 20.77 99.64 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 20.77 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.472

(partial model without unconserved sides chains): PDB file : Tito_3T1I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3T1I-query.scw PDB file : Tito_Scwrl_3T1I.pdb: