Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSLEEKTIAKEQIFSGKVIDLYVEDVELPNGKASKREIVKHPGAVAVLAVTDEGKIIMVKQFRKPLERTIVEIPAGKLEKGEEPEYTALRELEEETGYTAKKLTKITAFYTSPGFADEIVHVFLAEELSVLEEKRELDEDEFVEVMEVTLEDALKLVESREVYDAKTAYAIQYLQLKEALQAQK 5C7Q Chain:A ((1-173)) MKHLEEKTLSTRQIFKGRYLKIEQDQVQAPDGRTYTREYILHPGAAMMIPLLPNGNVVMIHQYRHAVKKVFLEFPAGKRDHNEETLLTAKRELLEETGYEAKDWKFLTTIHPVIGYSNEHIDLYLARDLTHLEQR--LDQGEFIEVVEVKPADLMQLVLEGKVSDVKTQIGAFWL----------

General information: TITO was launched using: RESULT: Template: 5C7Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 755 -59436 -78.72 -343.56 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : -78.72 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.448

(partial model without unconserved sides chains): PDB file : Tito_5C7Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5C7Q-query.scw PDB file : Tito_Scwrl_5C7Q.pdb: