Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMIKQCVICLSLLVFGTTAAHAEETPLVTARHMSKWEEIAVKEAKKRYPLAQVLFKQKVWDRKRKDEAVKQYHLTLREGSKEFGVFVTISFDPYSQKVNKIAILEEYQ 2LYX Chain:A ((4-87)) ------------------------TPLVTARHMSKWEEIAVKEAKKRYPLAQVLFKQKVWDRKRKDEAVKQYHLTLREGSKEFGVFVTISFDPYSQKVNKIAILEEYQ

General information: TITO was launched using: RESULT: Template: 2LYX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 328 -16524 -50.38 -196.71 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.87 3D Compatibility (PKB) : -50.38 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.347

(partial model without unconserved sides chains): PDB file : Tito_2LYX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2LYX-query.scw PDB file : Tito_Scwrl_2LYX.pdb: