Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDEIWFAGIDLGGTTIKLAFINQYGEIQHKWEVPTDK-TG-DTITVTIAKTIDSKLDELQKPKHIIKYIGMGAPGPVDMAAGVVYETVNLGWKN-YALKNHLETETGIPAVIENDANIAALGEMWKGAGDGAKDVILVTLGTGVGGGIIANGEIVHGINGAGGEIGHICSIPEGGAPCNCGKTGCIETIASATGIVRIAKEKIANAKK-TT--R-LKATEQLSARDVFEAAGENDEIALEVVDYVAKHLGLVLGNLASSLNPSKIVLGGGVSRAGELLRSKVEKTFRKCAFPRAAQAADISIAALGNDAGVIGGAWIAKNEWLKHQNC 2QM1 Chain:A ((4-322)) -MDKKIIGIDLGGTTIKFAILTTDGVVQQKWSIETNILEDGKHIVPSIIESIRHRIDLYNMKKEDFVGIGMGTPGSVDIEKGTVVGAYNLNWTTVQPVKEQIESALGIPFALDNDANVAALGERWKGAGENNPDVIFITLGTGVGGGIVAAGKLLHGVAGCAGEVGHVTVDPN-GFDCTCGKRGCLETVSSATGVVRVARHLSEEFAGDSELKQAIDDGQDVSSKDVFEFAEKGDHFALMVVDRVCFYLGLATGNLGNTLNPDSVVIGGGVSAAGEFLRSRVEKYFQEFTFPQVRNSTKIKLAELGNEAGVIGAASLALQF-------

General information: TITO was launched using: RESULT: Template: 2QM1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1771 37254 21.04 119.40 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : 21.04 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.544

(partial model without unconserved sides chains): PDB file : Tito_2QM1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2QM1-query.scw PDB file : Tito_Scwrl_2QM1.pdb: