Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITQLYRERTAADLKNRISKVLLNGNETEIVELTIQGAVVTVLTQREEDIKHIKSVQILDDQNNVITERTTDLDVSNNRTLDFRITFEVV 2PH0 Chain:A ((120-134)) ----------------------------------------------------TASVVFFNANGAAMF-----------------------

General information: TITO was launched using: RESULT: Template: 2PH0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 13 -4150 -319.23 -276.67 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : -319.23 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.683

(partial model without unconserved sides chains): PDB file : Tito_2PH0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2PH0-query.scw PDB file : Tito_Scwrl_2PH0.pdb: