Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKIESTQELEKAVK---DDWSVFMFSADWCPDCRFVEPFLPELEANFPEFTYYYVDRDKFIDTCAEWEIYGIPSFVVFNEGKEVNRFVSKDRKTKEEIEQFLTDSLAKA 4POM Chain:B ((6-102)) VKQIESKTAFQKALKAAGDKLVVVDFSATWCGPCKMIKPFFHSLSEKYSNVIFLEVDVDDCQDVASECEVKCMPTFQFFKKGQKVGEFSGANKKKLE-------------

General information: TITO was launched using: RESULT: Template: 4POM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 389 -11100 -28.53 -118.08 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : -28.53 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.677

(partial model without unconserved sides chains): PDB file : Tito_4POM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4POM-query.scw PDB file : Tito_Scwrl_4POM.pdb: