Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYEFKDYYQNTVQLSFDDQPFSDSPKHVWVICRFGGKWLLTEHEDRGYEFPGGKVEPMECAEEAALREVKEETGARVKSLKYLGQYKVLGKEKVIVKNIYFADIEKLEKQADYFETKGPVLFHELPENLSRNKKFSFIMKDSVLPISLKKLKESGWIE 4JZS Chain:D ((21-178)) MYEFKDYYQNTVQLSFDDQPFSDSPKHVWVICRFGGKWLLTEHEDRGYEFPGGKVEPMECAEEAALRAVKEETGARVKSLKYLGQYKVLGKEKVIVKNIYFADIEKLEKQADYFETKGPVLFHELPENLSRNKKFSFIMKDSVLPISLKKLKESGWIE

General information: TITO was launched using: RESULT: Template: 4JZS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 801 -71314 -89.03 -451.35 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain D : 1.00 3D Compatibility (PKB) : -89.03 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 1.00 QMean score : 0.524

(partial model without unconserved sides chains): PDB file : Tito_4JZS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4JZS-query.scw PDB file : Tito_Scwrl_4JZS.pdb: