Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNFDKREERLGTQSVKWDKTGELFGVTDALPMWVADMDFRAPEAITEALKERLDHGIFGYTTPDQKTKDAVCGWMQNRHGWKVNPESITFSPGVVTALSMAVQAFTEPGDQVVVQPPVYTPFYHMVEKNGRHILHNPLLE-KDGAYAIDFEDLETKLSDPSVTLFILCNPHNPSGRSWSREDLLKLGELCLEHGVTVVSDEIHSDLMLYGHKHTPFASLSDDFADISVTCAAPSKTFNIAGLQASAIIIPDRLKRAKFSASLQRNGLGGLNAFAVTAIEAAYSKGGPWLDELITYIEKNMNEAEAFLSTELPKVKMMKPDASYLIWLDFSAYGLSDAELQQRMLKKGKVILEPGTKYGPGGEGFMRLNAGCSLATLQDGLRRIKAALS 4DGT Chain:B ((7-391)) -NFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAAPCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLYRRHNWKIKSEWLIYSPGVIPAISLLINELTKANDKIMIQEPVYSPFNSVVKNNNRELIISPLQKLENGNYIMDYEDIENKIKD--VKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMASISKEFEKNTITCMAPTKTFNIAGLQSSYVVLPDEKDYKLLDDAFTRIDIKRNNCFSLVATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGLSDEELESILVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIN

General information: TITO was launched using: RESULT: Template: 4DGT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2142 -64117 -29.93 -166.97 target 2D structure prediction score : 0.79 Monomeric hydrophicity matching model chain B : 0.84 3D Compatibility (PKB) : -29.93 2D Compatibility (Sec. Struct. Predict.) : 0.79 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.644

(partial model without unconserved sides chains): PDB file : Tito_4DGT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4DGT-query.scw PDB file : Tito_Scwrl_4DGT.pdb: