Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MELYIITGASKGLGQAIALQA---LEKGHEVHALSRTKTDVSHKK--LTQHQIDL-INLEEA----EQQFETLLSSI-DSDRYSGIT---LINNAGMVTPIKRA--GEASLDELQRHYQLNLTAPVLLSQLFTKRFASYSG-KKTVVNITSGAAKNPYKGWSAYCSSKAGLDMFTRTFGFEQEDEELPVNMISFSPGVMDTEMQAVIRSSSKKDFHHIERFRKLNETGSLRSPDFIAGTLLSLLEKGTENGRIYDIKEFL 1NAS Chain:A ((8-248)) --VCVLTGASRGFGRALAPQLARLLSPGSVMLVSARSESMLRQLKEELGAQQPDLKVVLAAADLGTEAGVQRLLSAVRELPRPEGLQRLLLINNAATLGDVSKGFLNVNDLAEVNNYWALNLTSMLCLTSGTLNAFQDSPGLSKTVVNISSLCALQPYKGWGLYCAGKAARDMLYQVLAAEEPS----VRVLSYAPGPLDNDMQQLARETS-KDPELRSKLQKLKSDGALVDCGTSAQKLLGLLQKDT------------

General information: TITO was launched using: RESULT: Template: 1NAS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1026 25060 24.42 111.87 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : 24.42 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.450

(partial model without unconserved sides chains): PDB file : Tito_1NAS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1NAS-query.scw PDB file : Tito_Scwrl_1NAS.pdb: