Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRYMKLFFLVTDIGFILYWLSAGFSLIPESWAFK----HHDHPFMIAWN------WSFFPLDILISVTGLYSLYLQRVNRADWKLMALISLVLTCCSGLQALSFWTFSSDFDLVWWLFNGYLLIYPLFFIHLLLKEGRRTKQS 2YEV Chain:B ((38-111)) -----LFPWVYFFSFLIFLVVAG-SLAYVTWKFRARPEDQEEPPQIHGNDRLEVVWTLIPLAIVFVLFGLTAKALIQVNR---------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2YEV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 36 -4925 -136.79 -76.95 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.57 3D Compatibility (PKB) : -136.79 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.57 QMean score : -0.157

(partial model without unconserved sides chains): PDB file : Tito_2YEV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YEV-query.scw PDB file : Tito_Scwrl_2YEV.pdb: