Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLNLQGKTALVTGSTSGIGKAIASSLAEEGAAVIINGRREEKVNQTIDELKTQHAEAVLYPAAFDLGTEEGCNELFQAYPEVDILVNNLGIFEPAEYFDIPDDEWFRFFEVNIMSGVRLTRRYLHNMIEKKEGRVIFIASEAAIMPSQEMAHYSATKTMQLSISRSLAELATGTNVTVNTVMPGSTLTEGVETMLNSLYPGENLTVQEAEARFMKENRPTSIIQRLIRPEEIAHFVTFLSSPLSSAINGAALRADGGLVRSVF 3T4X Chain:A ((5-264)) ----LKGKTALVTGSTAGIGKAIATSLVAEGANVLINGRREENVNETIKEIRAQYPDAILQPVVADLGTEQGCQDVIEKYPKVDILINNLGIFEPVEYFDIPDEDWFKLFEVNIMSGVRLTRSYLKKMIERKEGRVIFIASEAAIMPSQEMAHYSATKTMQLSLSRSLAELTTGTNVTVNTIMPGSTLTEGVETMLNSLYPNEQLTIEEAEKRFMKENRPTSIIQRLIRPEEIAHLVTFLSSPLSSAINGSALRIDGGLVRSVF

General information: TITO was launched using: RESULT: Template: 3T4X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1363 -138318 -101.48 -531.99 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.96 3D Compatibility (PKB) : -101.48 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.96 QMean score : 0.544

(partial model without unconserved sides chains): PDB file : Tito_3T4X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3T4X-query.scw PDB file : Tito_Scwrl_3T4X.pdb: