Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKNGELKPGDKLDSVQALAESFQVSRSAVREALSALKAMGLVEMKQGEGTYLKEFELNQISQPLSAALLMKKEDVKQLLEVRKLLEIGVASLAAEKRTEADLERIQDALKEMGSIEADGELGEKADFAFHLALADASQNELLKHLMNHVSSLLLETMRETRKIWLFSKKTSVQRLYEEHERIYNAVAAGNGAQAEAAMLAHLTNVEDVLSGYFEENVQ 3C7J Chain:A ((38-232)) AIIDGSLPSGTALR-QQELATLFGVSRMPVREALRQLEAQSLLRVETHKGAVVAP--------------L-ITEDAVDAYALRILLESEALRLSIPLLDADDLAAAASYIEQLEVE-TDFGQIGRLNRMFHLSLYAKTHNKRLMRLVEEGLNEEERFLR-F--NLS-S-MGLGKLSQDDHWQLLRLAEQKAVEPCVEALQYHLNRGVQAVTQYLQSQ--

General information: TITO was launched using: RESULT: Template: 3C7J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 689 -66233 -96.13 -339.65 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -96.13 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.602

(partial model without unconserved sides chains): PDB file : Tito_3C7J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3C7J-query.scw PDB file : Tito_Scwrl_3C7J.pdb: