Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGQKPKIAIFGGGTGLSVLLRGLKHKPVDITAIVTVADDGGSSGRLRNELKIPPPGDIRNVLAALSDVEPLVEDLFQHRFNKGNDLTGHSLGNLILAAMTNITGDFFHAVTEMSKVLNVRGKVLPAANASVVLHAEMEDGRVVSGESTIPEYGQRIKRVFLTPEQIDPLPETIDVIREADLIIIGPGSLYTSILPNLLVPKIGEEVIKAPAKKVYICNVMTQPGETLHYTAADHVKALNQHMGCGFIDTILVNSEDIPDEIKRKYEMESARPVDFDIEELKAMGLEVIRDQIVTYKNDVIRHDTHKVASLLVDLLKE 2O2Z Chain:A ((1-313)) GMKKKNVIVFGGGTGLSVLLRGLKTFPVSITAIVTVADDGGSSGRLRKELDIPPPGDVRNVLVALSEVEPLLEQLFQHRFENG-GLSGHSLGNLLLAGMTSITGDFARGISEMSKVLNVRGKVLPASNRSIILHGEMEDGTIVTGESSIPKAGKKIKRVFLTPKDTKPLREGLEAIRKADVIVIGPGSLYTSVLPNLLVPGICEAIKQSTARKVYICNVMTQNGETDGYTASDHLQAIMDHCGVGIVDDILVHGEPISDTVKAKYAKEKAEPVIVDEHKLKALGVGTISDYFVLEQ--VLRHNASKVSEAILE----

General information: TITO was launched using: RESULT: Template: 2O2Z.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1790 -227933 -127.34 -735.27 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.91 3D Compatibility (PKB) : -127.34 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.91 QMean score : 0.657

(partial model without unconserved sides chains): PDB file : Tito_2O2Z.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2O2Z-query.scw PDB file : Tito_Scwrl_2O2Z.pdb: