Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITKRIIPCLDVKEGRVVKGIQFLELKDAGDPVELAEVYDREGADELVFLDISASHEGRKTMVDVVEQVAAKLAIPFTVGGGINQLSDMKTILRAGADKVSVNTAAVLRPELITEGAEFFGSQCIVLAIDAKYDKESDTYKVYTHGGRKKTDWEVTAWAKEGVKRGAGEILLTSMDSDGEKKGFDHTLTKLVSEAVPVPVIASGGAGNAQHMLEAFTKGEADAALAASIFHYKETSIKEVKSYLKEYGVNVR 4EVZ Chain:A ((1-250)) MLAKRIIPCLDVKDGRVVKG----NLRDAGDPVELAARYDEEGADELVFLDITASHEGRETMLEVVERTAEQVFIPLTVGGGIRSVEDASRLLRAGADKVSINTAAVKNPELITEAAEEFGSQAVVVAIDAK--RVGGGWEVFTHGGRKPTGLDAVEWARKVVELGAGEILLTSMDRDGTKAGYDLELTRAVSEAVSVPVIASGGAGELEHFAEVFELEGADAALAASIFHFGEITIREVKAYLRERGIEVR

General information: TITO was launched using: RESULT: Template: 4EVZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1479 -133194 -90.06 -541.44 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.89 3D Compatibility (PKB) : -90.06 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.89 QMean score : 0.612

(partial model without unconserved sides chains): PDB file : Tito_4EVZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EVZ-query.scw PDB file : Tito_Scwrl_4EVZ.pdb: