Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRKKELFDFTNITPKLFTELRVADKTVLQSFNFDEKNHQIYTTQVASGLGKDNTQSYRITRLSLEGLQLDSMLLKHGGHGTNIGIENRNGTIYIWSLYDKPNETDKSELVCFPYKAGATLDENSKELQRFSNMPFDHR-VTPALDMKNRQLAIRQYDTKNNNNKQWVTIFNLDDAIANKNNPLYTINIPDELHYLQGFFLDDGYLYWYTGDTNSKSYPNLITVFD--SDNKIVLQKEITVGKDLSTRYENNFREPEGICMYTNPETGAKSLMVGITSGKEGNRISRIYAYHSYENFMNHVPMLRSPLLKTVGHQDTPPERFQPFIQTFILEYNAQNKKWMVPTSGYLPSYTSNLVRNITINADGNLQVTLNERYISLLHQSIEGDFRLKQKDIRMGSWYFAGGEKSNVLEIGFMKGSTKIRPDDAAISNASRMSIFMIVADKIEV 1RI6 Chain:A ((2-334)) ----------------------------------SLKQTVYIASPESQ-------QIHVWNLNHEGALTLTQVVDVPGQVQPMVVSPDKRYLYVGV-------RPEFRVLAYRI------APDDGALTFAAESALPGSLTHISTDHQGQFVFVGSYNAG------NVSVTRLED-----GLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQ----DRICLFTVSDDGHLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKD--------------------------PHGNIECVQTLDMMPENFSDTRWAADIHITPDGRHLYACDRT------ASLITVFSVSEDGSVLSKEGFQPTETQPRGFNVDHS--------GKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAHE---

General information: TITO was launched using: RESULT: Template: 1RI6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2162 -3835 -1.77 -11.62 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -1.77 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.350

(partial model without unconserved sides chains): PDB file : Tito_1RI6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1RI6-query.scw PDB file : Tito_Scwrl_1RI6.pdb: