Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNFYRVEQMPGFIKTEMQKIQKAVQPFMKKTVIYRFLAIPLAAFSLFNL--------------AAFLFHA---SADRESLISAGIFALLAALGLAFFKEAGYQHKQIQKTVHIYMLNRIKKSEILSEERKSSYARQIKEEPFAMRSFVEFLTEEDRRKKMY 3EJD Chain:B ((105-227)) ------ESYQPYIIETVHHLLDQVQGKKKMEVISDF-AFPLASFVIANIIGVPEEDREQLKEWAASLIQTIDFTRSRKALTEGNIMAVQA---MAYFKELIQKRKRHPQQDMISMLLKG---DKLTEEEAAS-----------------------------

General information: TITO was launched using: RESULT: Template: 3EJD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 198 -8743 -44.16 -85.72 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain B : 0.69 3D Compatibility (PKB) : -44.16 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.184

(partial model without unconserved sides chains): PDB file : Tito_3EJD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EJD-query.scw PDB file : Tito_Scwrl_3EJD.pdb: