Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNHGYRTIEFLGLTFNLTNILMITVASVIVLLIAILTTRTLSIRPGKAQNFMEWIVDFVRNIIGSTMDLKTGANFLALGVTLLMYIFVSNMLGL-PFSITIGHELWWKSPTADPAITLTLAVMVVALTHYYGVKMKGLKEYSKDYL-------RPVPFMLPMKIIEE----FANTLTLGLRLYGNIFAGEILLGLLAGLATSHYSQSVALGLVG-TIGAILPMLAWQAFSLFIGAIQAFIFTMLTMVYMSHKISHDH 5FIK Chain:W ((14-225)) -------------------ILGLPLVTLIVLFPSLLFPTSNRLVSNRFVTLQQWMLQLVSKQMMSIHNSK-GQTWTLMLMSLILFIGSTNLLGLLPHSFT---------PTTQLSMNLGMAIPL-----WAGAVITGFRNKTKASLAHFLPQGTPTP-LIPMLVIIETISLFIQPMALAVRLTANITAGHLLIHLIGGATLALMSISTTTALITFTILILLTILEFA-----VAMIQAYVFTLLVSLYL-----HDN

General information: TITO was launched using: RESULT: Template: 5FIK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain W - contact count / total energy / energy per contact / energy per residue : 733 -116304 -158.67 -584.44 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain W : 0.73 3D Compatibility (PKB) : -158.67 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.326

(partial model without unconserved sides chains): PDB file : Tito_5FIK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FIK-query.scw PDB file : Tito_Scwrl_5FIK.pdb: