Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNELKQTWKTMLSEFQDQAELKQDQLFVLGCSTSEVAGSRIGTSGSVDIAESIYSGLAELREKTGIHLAFQCCEHLNRALVVEAETAKLFRLPTVSAVPVPKAGGAMASYAFKQMKSPVLVETIQAD--AGIDIGDTFIGMHLKPVAVPVRVSQNSLGSAHVTLARTRPKLIGGVRAVYECE----------- 1V8D Chain:A ((40-232)) MEGIRRAAQRAAEEFLQAFPMAPGSLFVLGGSTSEVLG----TRPSLEAAHAVLEGLLPPLLERGVHVAVQACEHLNRALVVERETARAFGKEEVAVFPHPKAGGAKATAAFLRFRDPVMVESLKAQAHGGMDIGGVLIGMHLRPVAVPLRLSVRKIGEAVLLAAKTRPKLVGGARAVYTREEMLKKLEEFLP

General information: TITO was launched using: RESULT: Template: 1V8D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 993 -99398 -100.10 -564.76 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -100.10 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.544

(partial model without unconserved sides chains): PDB file : Tito_1V8D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1V8D-query.scw PDB file : Tito_Scwrl_1V8D.pdb: