Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MANEFRVCDDCQAT-NIKTLLPRLKEVDPDAKVEVGCQSYCGPGRKKSFAFVNNRPLSAP-------TEDELIDKVKKKIKK 2LJK Chain:A ((26-100)) -RIVVEYCEPCGFEATYLELASAVKEQYPGIEIESR-LG----GTGAFEIEINGQLVFSKLENGGFPYEKDLIEAIRRASN-

General information: TITO was launched using: RESULT: Template: 2LJK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 214 3627 16.95 54.13 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 16.95 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.708

(partial model without unconserved sides chains): PDB file : Tito_2LJK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2LJK-query.scw PDB file : Tito_Scwrl_2LJK.pdb: