Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDEQKKPEQIHRRDILKWGAMAGAAVAIGASGLGGLAPLVQTAAKPSKKDEKEEEQIVPFYGKHQAGITTAHQTYVYFAALDVTAKDKSDIITLFRNWTSLTQMLTSGKKMSAEQRNQYLPPQDTG-ESADLSPSNLTVTFGFGPGFFEKDGKDRFGLKSKKPKHLAALPAMPNDNLDEKQGGGDICIQVCADDEQVAFHALRNLLNQAVGTCEVRFVNKGFLSG----GKNGETPRNLFGFKDGTGNQSTKDDTLMNSIVWIQSG--EPDWMTGGTYMAFRKIKMFLEVWDRSSLKDQEDTFGRRKSSGAPFGQKKETDPVKLNQIPS------NSHVSL---AKSTGKQILRRAFSYTEGLDPKTGYMDAGLLFISFQKNPDNQFIPMLKALSAKDALNEYTQTIGSA-LYACPGGCKKGEYIAQRLLES 2Y4D Chain:B ((28-401)) --------------------------------------------------DARNEKQ--PFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLTQGGA-APETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFD----ERFGLAPQMPKKLQKMTRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHAARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANP--SESSLMLRRGYSYSLGVT-NSGQLDMGLLFVCYQHDLEKGFLTVQKRLNG-EALEEYVKPIGGGYFFALPGVKDANDYFGSALL--

General information: TITO was launched using: RESULT: Template: 2Y4D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1895 7732 4.08 21.90 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : 4.08 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.399

(partial model without unconserved sides chains): PDB file : Tito_2Y4D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Y4D-query.scw PDB file : Tito_Scwrl_2Y4D.pdb: