Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----MKVRFLQISDLHF----------QFQNYDT------IVMRDKLLDFIEVLRRESDFDFLLLTGDIAHKGDVYNEDVKEYLNGIIKSMGLSKNNVHLVPGNHDISRDMTRTLLIDSIMKSPNPSEMLDKLDQKATNILVEGQRKFFDFYEDFMGVKYPEEDLHFLYQSEKYNVLSINTCLLSDKKGEEGTLLTAQM--KLYKAIRKMNKEKNKGKVLNIAIGHHTLGCIESSE------RESIKAHFDDYFIDLYLAGHVHDSSFNITANTNENPFLELVSGAIIKDEYATPEFISVDVNLDNGETEVTYYIWNTEYKYWSKNNQGGRRLQEGKLNYKINRLESLIEKEIEDNDDKIDEDEFKSFIIDFHEYRESYKTFTSNFDNQIGLDKKFYDMKSGETFKRKFDSYSEYFGVINHIMDSTSYVSADKKELIAETIVDKYLEFHNQYNNGDEIFVKIVNEIYLECHSVLPYSKALTKKYIKILTCWCIYECEIFNDNKRSVKND 2XMO Chain:A ((33-443)) PIEKDRNLSMVVTTDVHYFAPSLTDNGKAFEKYVAAGDGKQLAYSDEITDAFLADVESKKTDVLIISGDLTNNGE---KTSHEELAKKLTQVEKNGTQVFVVPGNHDINNPWARKFEKDKQLPTDTIS-------------PTDFSKIYSDFGYEDAISSDEFSLSYLAAPSSKVWLLMLDTAIYKTNMQQGNPTTEGGLTAGTLDWIKESSALAKKNGAKLIPVLHHNLTDHNDVKGYTINYNQQVIDALTEGAMDFSLSGHIHTQNIRSAKSTDGKEITDIVTNAL---SVFPHKYGNITYSAKNKNFTYQSQKLDMEAWAKAQGST----------DENLLNFDQFDYETFYNSGYDKAMMDLMT-------------------------------------------------------DESYDKYNQADKEKMADTMGLNNMYFFAGTAPPKSDGMALWDSA----------PNSFLKDYVLS-----SSNPPKKSNDYYVSP--

General information: TITO was launched using: RESULT: Template: 2XMO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2152 122264 56.81 321.75 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 56.81 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.367

(partial model without unconserved sides chains): PDB file : Tito_2XMO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2XMO-query.scw PDB file : Tito_Scwrl_2XMO.pdb: