Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEWNKTKSIFIVAFLILDIFLGYQFFQKWQATGKEYEVIKNDVEHDMKADHITYEGLNKEATEGYRITANQKSFSKEEIEALKDQKPLMDMPSDDHKVTSLKMKFANPIALSKKDIEDDAQALVSSKIQDGEKYKLWKVDKSKKEIIFFQTYEGHYIYQKTDNPSNMIGQVVLHLNGKNEVVSYDQTTLETFKQIQKESLITEMDAVELLYYQNQLKEYSTVKSCKFGYVAQYPLTSTQVLAPVWRITVEYEKKVNGEKKTVQEYFTVNALESTILDTDQ 2O3O Chain:A ((14-252)) ---------------------------------------KNDVEHDMKADHITYEGLNKEATEGYRITANQKSFSKEEIEALKDQKPLMDMPSDDHKVTSLKMKFANPIALSKKDIEDDAQALVSSKIQDGEKYKLWKVDKSKKEIIFFQTYEGHYIYQKTDNPSNMIGQVVLHLNGKNEVVSYDQTTLETFKQIQKESLITEMDAVELLYYQNQLKEYSTVKSCKFGYVAQYPLTSTQVLAPVWRITVEYEK----EKKTVQEYFTVNALESTILDT--

General information: TITO was launched using: RESULT: Template: 2O3O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1082 -78664 -72.70 -334.74 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.91 3D Compatibility (PKB) : -72.70 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.91 QMean score : 0.521

(partial model without unconserved sides chains): PDB file : Tito_2O3O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2O3O-query.scw PDB file : Tito_Scwrl_2O3O.pdb: