Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLKMRNLISNYELAKDLLKNWEYDEENLDEMLSYFRISSNAIYPFFYKKKICFLRIAPTDEKRKENIYGEIEFIQYLR-EHDFKALCPVPSKNGEFIRIVNTKWGEY---YATVFEKVAGKPVEACEYSEKMYHEYGKTMGRMHRLS------SGFK-------------PIIKKWTHEDVLNEIETMLTLYHCSNKAKIELENLRVELAKLPKNHLSYGLIHYDFELDNVFYDEKT--NTCSVIDFDDGMYHWYSTDIEQFLDSVSEEKGELEAEQVKIAFFEGYQS--EYPIVEETKNSIPLMRRFINLYSYVRIHHCLSDSFTSEKEWMTELRVKLTNMLKSIEETW 6EF6 Chain:A ((27-301)) ---------DVTIAQQALTHYDVSD---NASLRLLNLSENATYLVEDGEHQSILRVHRQDYHQPHEIESELDWLAALRTDSDVTVPTVVPARDGRRVVTVDPAD---VPRHVVHFEMVGGAEPDEESLTLDDFQTLGRITASLHEHSQRWTRPAGFGRFSWDWEHCLGDTPRWGRWQDAEGVGASETALL-----TRAQDLLHRKLEEYGSGPDR---YGLIHADLRLANLLVDSSTPQRTITVIDFDDCGFGWYFYD---FGTAVSFIEHDPRLGEWQESWVAGYRSRRELPAADEA----------------------------------------------------

General information: TITO was launched using: RESULT: Template: 6EF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1084 -16685 -15.39 -68.10 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -15.39 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.367

(partial model without unconserved sides chains): PDB file : Tito_6EF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6ef6-query.scw PDB file : Tito_Scwrl_6EF6.pdb: