Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------MEDKKMLHILNNLYNINFKTMEFLREGGCISYRLSSEN-SSYFMKIISSAF--MDTAKQSLSIISYLTQKGFPSPRIIHTKEGLPYVEIEELNKKTLIVLFEFIEGSEPNE--GEDIETIGKLVGQLHNILKGY--KEPLPVHGKEFFIDRYIKILEQKNYDQNKIDTFREYGDVLWENVKN-----LPHGFCHGDLHRGNLLKTTTKKYYLLDFDTSSYAFPMYDIMVMCNSTDYFNFNEIGYQKSKNTYETFLKGYTKYRSLSKDELNSFYDFIAIYHYQLQATIIEIYGLNCVDI--EFLDNQLNW---LMKWREQCKAR----------- 1ZYL Chain:A ((4-328)) SAFTFQTLHPDTIMDALFEHGIRVDSGLTPLNSYENRVYQFQDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNH-----QGFYFAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLFEDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWR--DGPMFVDLDDARNGPAVQDLWMLLNGD---------KAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVYYLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY

General information: TITO was launched using: RESULT: Template: 1ZYL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1330 39593 29.77 136.53 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 29.77 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.337

(partial model without unconserved sides chains): PDB file : Tito_1ZYL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1zyl-query.scw PDB file : Tito_Scwrl_1ZYL.pdb: