Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWQAISHLLNEQLGEGDIELRNELPGGEIHAAWHLRFAGRDLFVKCDERELLPIFTAEADQLELLSRSKTVSVPKVWALGSD-RDYSFLVMDYLPARPLDA-------HNAFLLGQQIARLHQWSDQPQ--FGLDFDNDLSTTPQPNAWQRRWSTFFAEQRIGWQLELAAEKG-LAFGNIDAIVDHVHQRLASHQPQPSLLHGDLWSENCALGPN-GPYIFD-PACFWGDRECDLAMLPLHP---EQPPQIYDGYQAVSPLPPDFLDRQPIYQLYTLFNRAILFGGEHLVNAQRALDRVLAA 3W0S Chain:A ((8-275)) -ATSVEKFLIEKFD--SVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCA--DGFYKDRYVYRHFAS-AALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGP-------QGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELA-GSPRLRAYMLRIGLDQLY--------------------

General information: TITO was launched using: RESULT: Template: 3W0S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1102 -41048 -37.25 -162.89 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -37.25 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.352

(partial model without unconserved sides chains): PDB file : Tito_3W0S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3w0s-query.scw PDB file : Tito_Scwrl_3W0S.pdb: