Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSPIVIKPDLAIDLATAGAVVARTGVDGQIAAISLLYGGEISAVYE-LALAGGLPPLVLKIYPDSMSWKMRKEARMLDLVRDRLPVPAPRLHLADASRDLIGLDYVVMSRLAGDRLGPLEQQLPPPLRQSAFRQVGRLSRAFHDIAMEAFGYIGPDGVWTAHADNRTYLTVQFDRKLTEFSSRGGAPDLALRIARHVEACSGLFEACAAPVLCHNDLHPGNILARADDDTVTLTGVVDFEGALAGDPLKDVAKALYYL----SAGQREAFLEGYGPMTRTEV---GATLDIYRLLFSLELWCWYAQFDATDKLAPLIADMELLLRSQ 3CSV Chain:A ((4-274)) -----------SREDEIRDFLA-T-HGYADWNRTP--------RYQRLRSPTGA-KAVLMDWSPEEGGDTQPFVDLAQYLRN-LDISAPEIYAEEH-----ARGLLLIEDLGDALFTEVINNDP-AQEMPLYRAAVDLLIHLHDAQTPELARLDP----ETLSEMTRLAFSEYRYAI---LGD-AAEDNRKRFEHRFAQILSA-QLEGDMVFVHRDFHAQNLLWLPEREGLARVGVIDFQDAKLGHRAYDLVSLLQDARRDVPAQVEAQMIDHYIQATGVDESHFRSAYAVIAVQRNMRIL--------------------------

General information: TITO was launched using: RESULT: Template: 3CSV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1115 -5922 -5.31 -23.22 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -5.31 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.275

(partial model without unconserved sides chains): PDB file : Tito_3CSV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3csv-query.scw PDB file : Tito_Scwrl_3CSV.pdb: