Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEDKKMLHILNNLYNIN--FKTMEFLREGG-CISYRLSSENSSYFMKIISSAF---MDTA-KQSLSIISYLTQKGFPSPRIIHTKEGLPYVEIEELNKKTLIVLFEFIEGSEPN----EGEDIETIGKLVGQLHNILKGYKEPLP--V-HGK---EFFIDRYIKILEQK----NYDQN-KIDTFREYGDVLWE---NV--KNLPHGFCHGDLHRGNLLKTT--TKKYYLLDFDTSSYAFPMYDIMVMCNSTDYFNFNE----IGYQKSKNTYETFLKGYTK--YRSLSKDELNSFYDFIAIYHYQLQATIIEIYGLNCVDIEFLDNQLNWLMKWREQCKAR 4OCV Chain:A ((24-318)) -TNEALFD-VASHFALEGTVDSIEPYGDGHINTTYLVTTDGPRYILQRMNTGIFPDTVNLMRNVELVTSTLKAQGKETLDIVRTTSGDTWAEID----GGAWRVYKFIEHTMSYNLVPNPDVFREAGRAFGDFQNFLSGFDANQLTETIAHFHDTPHRFEDFKKALAADELGRAAGCGPEIEFYLSHADQYAVVMDGLRDGSIPLRVTHNDTKLNNILMDATTGKARAIIDLDTIMPGSMLFDFGDSIRFGASTALEDERDLDKVHFSTELFRAYTEGFVGELRDSITAREAELLPFSGNL----------------------------------------

General information: TITO was launched using: RESULT: Template: 4OCV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1063 14982 14.09 57.62 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 14.09 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.425

(partial model without unconserved sides chains): PDB file : Tito_4OCV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ocv-query.scw PDB file : Tito_Scwrl_4OCV.pdb: