Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSPIVIKPDLAIDLATAGAVVARTGVDGQIAAISLLYGGEISAVYELALAGGLPPLVLKIYPDSM---SWKMRKEARMLDLVRDRLPVPAPRLHLADASRDLIGLDYVVMSRLAGDRLGPLEQQLPPPLRQSAFRQVGRLSRAFHDIAMEAFGYIGPD---------GVWTAHADNRTYL-TVQFDRKLTEFSSRGGA--PDLALRIARHVEACSGLFEACAAPVLCHNDLHPGNILARADDDTVTLTGVVDFEGALAGDPLKDVAKALYYLSAGQREAFLEGYGPMTRTE--VGATLDIYRLLFSLELWCWYAQFDATDKLAPLIADMELLLRSQ 3F7W Chain:A ((2-287)) -------------VNSVAARVT-ELTGREVAAVAERGHSHRWHLYRVELADGT-PLFVKALPDDAPALDGLFRAEALGLDWLGRSFGSPVPQVAGWD-------DRTLAMEWVDERPPTP-----------EAAERFGHQLAAMHLAGAESFGATWDGYIGPLPMDNTPRSTWPEFYAEQRILPYLRRAAD-RGALTPGDVRLVEKVLDALDH---LAGDPEPPARIHGDLWNGNVLWQD--D--G-AVVIDPA-AHGGHREADLAM-LALFGLPYLDRVRDAYNEVAPLAEGWRARIPLHQLHPLLVHVCLFG----AAYRTTLVDTARAALR--

General information: TITO was launched using: RESULT: Template: 3F7W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1440 -69788 -48.46 -259.43 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -48.46 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.274

(partial model without unconserved sides chains): PDB file : Tito_3F7W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3f7w-query.scw PDB file : Tito_Scwrl_3F7W.pdb: