Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDESGGEARAYMLGSGIVVKTQRPHRLRPRTSLEKEAHVLSLLAGPLAGRIPRLFGYGREDTAAGPVEFLVMSRIRGCAALHAGL-----PAREALLASLADVLRSVHAADVTGLRDSGLL----PADVDSAALRGRLQLGFADACDGFAAHRDRYAGAGAPERIAIAAVDALAG--LTDESPVLLHSNPGPTHV-FVGDGGLCTGLIDFGDAYASHPALDLRSWPDPADRIVLHEAYTGGRPTTRGFDAVWTVVMILADLNAMLHRADLAEAAGRDLALRLAQL 4DTB Chain:A ((30-232)) ---EGNDCIAYEINRDFIFKF--PKHSRGSTNLFNEVNILKRIHNKLPLPIPEVVFTGMP-SETYQMSFAGYTKIKGVPLTPLLLNNLPKQSQNQAAKDLARFLSELHSINISGFKSNLVLDFREKINEDNKKIKKLLS---------------RELKGPQMKKVDDFYRDILENEIYFKYYPCLIHNDFSSDHILFDTEKNTICGIIDFGDAAISDPDNDFIS-------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4DTB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 686 -20102 -29.30 -105.24 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -29.30 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.237

(partial model without unconserved sides chains): PDB file : Tito_4DTB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4dtb-query.scw PDB file : Tito_Scwrl_4DTB.pdb: