Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPTLFEGETPVDLDTVGSFVAQQCPQWGHLPLRAMTTSGTEHTTYRLG----DELLVRVPRDASSEAGLRKEIDWLPRLRDGVDLEMPVIEHVGAPSLDYPHTWTINRWVSGEDASARVLA-------GDVPSSWADVLAELVASLRAVDLREFAPHELPQGQRGGHVRCRVEALHGQTDPMSGPL-DPTPVMGLMEAALDA-GAPAPEPVLLHGDLIPGNLVVRNGRLSGLLDFGTLTTGYAAWDLTPAWWVLDRSGRERFRELLE-V-D---DVSWAWGRAFAASQGL--LADWVYAPR-GHALAPLGARAVAEALAD 5UXD Chain:A ((4-298)) ---------PEDLDALLDLAARHGLDLDGGTLRTEE-IGLDFRVAFARAHDGGDWVLRLPRRPDVLERAAVEGRLLAMLAPHLDVAVPDWRIST-------SELIAYPLLPGSPGLTVAADGEVSWHVDMASTVYARSLGSVVAQLHAVDAEA-AAATG--IEV-RSPAQVRGAWRQDLARVGAEFEIAPALRERWEAWLADDGCWPGHSVLTHGELYPAHTLVEDERITAVLDWTTAAVGDPAKDLMFHQVSAPSAIFEVALQAYAEGGGRPWPGLAR--HCTEMFSAAPLGYGLYALATGEAAHREAAAAALNPPE--

General information: TITO was launched using: RESULT: Template: 5UXD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1247 -27633 -22.16 -100.85 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -22.16 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.379

(partial model without unconserved sides chains): PDB file : Tito_5UXD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5uxd-query.scw PDB file : Tito_Scwrl_5UXD.pdb: