Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPTLFEGETPVDLDTVGSFVAQQCPQWGHLPLRAMTTSGTEHTTYRLG---DELLVRVPRDASS-EAGLRKEIDWLPRLRDGVDLEMPVIEHVGAPSL--------DYPHTWTINRWVSGEDASARVLAGDVPSSWADVLAELVASLRAVDLREFAPHEL-PQGQRGGHVRCRVE---ALHGQTDPM--SGP-L-DPTPVMGLMEAALDA-GAPAPEPVLLHGDLIPGNLVVRNG---RLSGLLDFGTLTTGYAAWDLTPAWWVLD-----RSGRERFRELLE----VDDVSWAWGRAFAASQGLLADWVYAPRGHALAPLGARAVAEALAD 6EF6 Chain:A ((27-320)) ------------DVTIAQQALTHYDVSDNASLRLLN--LSENATYLVEDGEHQSILRVHRQDYHQPHEIESELDWLAALRTDSDVTVPTVVPARDGRRVVTVDPAD-VPRHVVHFEMVGGAEPDEESLT----LDDFQTLGRITASLHEHSQRW-TRPAGFGRFSW--DWEHCLGDTPRWGRWQDAEGVGASETALLTRAQDLLHRKLEEYGSGPDRYGLIHADLRLANLLVDSSTPQRTITVIDFDDCGFGWYFYDFGTAVSFIEHDPRLGEWQESWVAGYRSRRELPAADEAMLPSFVFLRRLLLLAWMGS-------------------

General information: TITO was launched using: RESULT: Template: 6EF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1146 -13873 -12.11 -53.77 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -12.11 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.322

(partial model without unconserved sides chains): PDB file : Tito_6EF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6ef6-query.scw PDB file : Tito_Scwrl_6EF6.pdb: