Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQINPALAQAIEADSGFELTQVEFIGAGWFAQAYRFCSQAKHYVVRIS-KHYHDFLKDVYAYQH---WGQ-QLPIPAILAHGQFADGWAYAISPHIAGQTIVSLEAAELRQIQPALFK----SLQQLHQLKLGPVTGWGSVNSQGHGRYQSWAEEVLDIGNYKFDFDWHDWIKRDDQTGTLLRQGYRLMEQLLSTISTERSLI--HRDFGFDNVLCQPPTIVAVLDWADFGYGDWVYDIAQQSCYGQAEIYLQPWLKFAQQHDLIPDNFEKRLHCYWLRMNLTDLIVSQLRNDWHWQRKIVAELSWLIENPVVVRLNQ 1J7U Chain:A ((4-222)) MRISPELKKLIEKYRCVKDTE-----GMSPAKVYKLVGENENLYLKMTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVL-HFERHDGWSNLLMSEADG--VLCSEEYEDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSR-------LAELDYLLNNDLADVDCENW--EEDTPFKDPRELY----DFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1J7U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 840 -5247 -6.25 -25.23 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -6.25 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.178

(partial model without unconserved sides chains): PDB file : Tito_1J7U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1j7u-query.scw PDB file : Tito_Scwrl_1J7U.pdb: