Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKDIENRFGAKIQDIKVLKSGWAGEIISLEFKDNTQKYVIKTYNSSKNGLENIKQEWTGLNLLYNANYPVPRPIISDFVNEKPYIVMEKIEGENLWT-CYQTLSKEDRQQLLEKFVKVFLK-LHELDVSIIDKELRKDSTSSFIEKEI-------NEIKKLVEENKLEYFTQIIDWLQKEKANIIGEKLSIIHRDYHPWNVIVD-NNKAIYVIDLL-WGIGDYRFDLAWMYTLMERSGFEDFSQNALKKYKELKNQNINNFEYFKVFSTLRWLINVMISLKTGENLNETRNKEFENFVSPLIQNRIMSIKKITGIQITI 6CAV Chain:A ((27-255)) -----------KVDSIEIIGSGYD----SVAYLVNNE-YIFKTKF-----YAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEE-QNLLKRDIASFLRQMHGLDYTDI-SECTIDNKQNVLEEYILLRETIYNDLTD-IEKDYIESFMERLN-----ATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMY---------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 6CAV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 816 -3297 -4.04 -15.62 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -4.04 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.293

(partial model without unconserved sides chains): PDB file : Tito_6CAV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6cav-query.scw PDB file : Tito_Scwrl_6CAV.pdb: