Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQINPALAQAIEADSGFELTQVEFIGAGWFAQAYRFCSQAKHYVVRIS-KHYHDFLKDVYAYQH---WGQ-QLPIPAILAHGQFADGWAYAISPHIAGQTIVSLEAAELRQIQPALFK----SLQQLHQLKLGPVTGWGSVNSQGHGRYQSWAEEVLDIGNYKFDFDWHDWIKRDDQTGTLLRQGYRLMEQLLSTISTERSLI--HRDFGFDNVLCQPPTIVAVLDWADFGYGDWVYDIAQQSCYGQAEIYLQPWLKFAQQHDLIPDNFEKRLHCYWLRMNLTDLIVSQLRNDWHWQRKIVAELSWLIENPVVVRLNQ 3Q2J Chain:A ((4-222)) MRISPELKKLIEKYRCVKDTE-----GMSPAKVYKLVGENENLYLKMTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVL-HFERHDGWSNLLMSEADG--VLCSEEYEDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSR-------LAELDYLLNNDLADVDCENW--EEDTPFKDPRELY----DFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3Q2J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 829 -10375 -12.52 -49.88 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -12.52 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.210

(partial model without unconserved sides chains): PDB file : Tito_3Q2J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3q2j-query.scw PDB file : Tito_Scwrl_3Q2J.pdb: