Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHSRPQISPERAAQLAAEWFEGQLDAPAELDSYADRNFLVRAPDGTKAVLKVPNVELAEDIDLQIAILKWLEARPSAPLVPRVLGPTRTIEDDAGRPTRAWMVGWIEGELWFDASPTPALREELGAALGQLARDLEDFRHPGMERHFAWNLAEANWIAEELHRFEDPARAELVCDALMQFQGRVLPRLAELPTQVIHGDANDKNLAVAEGRLVGVFDFGDACWCPAVCDLAIALAYAAMDLRLGCALPEVAAACAQVCAGYHGRRPLTALELDLLYDLLRARLAVSVTSSTRAREAEPDNAYVSASEQGAWSVLEALGAVGRRAFTATVAEACGLALAPRVPGARTGDELLAARRRRLGPSLSLSYASSGMPLYIRRGEGSWLFDEHDQAFLDCVNNV--CHVGHCHPRVVEAGAAQMARLNTNTRYLHEGLVDYAEALCATLPAPLEVVYLVNSGSEANELALRLARDYTGGFDVAVLDAAYHGNTGNLVDMSPYKFDAPGGRGRREWVHVLPTPDPYRGAHGDDGPAYAAALDAADAAARGRGSRLAALFCESVLGCAGQVVPASGFLAAAYARARAAGAVCIADEVQVGFGRVGDGMWAFEAEGVVPDILTLGKPIGNGHPLGAVVTTRAIAEALGGG--RMEFFCTFGGNPVSAAVGAAVLAVIEDEGLVANARDTGSWLRGAFEQLAADPVLGRGIGEVRGRGLFIGVELVEDRST-----KRPDAARASAIVAHARARGVLLSTDGPARNVIKIKPPICFADVEARILASTLARALSATMR 4AO9 Chain:A ((69-447)) ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------FPLTIARGEGAALWDADGHRYADFIAEYTAGVYGHSAPEIRDAVIEAMQGGI-NLTGHNLLEGRLARLICERFPQ-IEQLRFTNSGTEANLMALTAALHFTGRRKIVVFSGGYHGGVLGFGARPSPTT-------VPFDF--LVLP------Y--------NDAQTARAQIERHGPEIAVVLVEPMQGASGCIPGQPDFLQALRESATQVGALLVFDEVMTS--RLA-PHGLANKLGIRSDLTTLGKYIGGGMSFGAFGGRADVMALFDPRTGPLAHSGTFNNNVMTMAAGYAGLTKLFTPEAAGALAERGEALRARLNALCAN---EGVAMQFTGIGSLMNAHFVQGDVRSSEDLAAVDGRLRQLLFFHLLNEDIYSSPRG----FVVL--SLPLTDADIDRYVAAIGSFIGGHG-

General information: TITO was launched using: RESULT: Template: 4AO9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2155 -63831 -29.62 -172.52 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -29.62 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.228

(partial model without unconserved sides chains): PDB file : Tito_4AO9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ao9-query.scw PDB file : Tito_Scwrl_4AO9.pdb: