Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPTLFEGETPVDLDTVGSFVAQQCPQWGHLPLRAMTTSGTEHTTYRLGDELLVRVPRDASSEAGLRKEIDWLPRLRDGVDLEMPVIEHVGAPSLDYPHTWTINRWVSGEDASARVLAGDVPSSWADVLAELVA-SLRAVDLREFAPHELPQGQRGGHVRCRVEALHGQTDPMSGPLDPTPVMGLMEAALDAGAPAPEPVLLHGDLIPGNLVVRNGR---LSGLLDFGTLTTGYAAWDLTPAWWVLDRSGRERFRELLEVDDVSWAWGRAFAASQGLLADWVYAPRGHALAPLGARAVAEALAD-------- 4WW7 Chain:A ((4-257)) ----------EFIDKVSSYL----------------TPDVDIAPISQGAAIVFTTTTHP-----------YLPRAKD-------------------SHQKYIIKYRPRTLNESRLLAKLYLIPGLCVPQLIACDPYNGFIWLEFLGEDLPGGHGFSNLK-NFLWMHDQ-DPYS-----DLVATTLRKVGRQIGLLHWNDYCHGDLTSSNIVLVRDGARWTPHLIDFGLGSVSNLVEDKGVDLYVLERAILSTHSK----------HAEKYNAWIMEGFEEVYREQG-AKGAKKLKEVTKRFEEVRLRGRKR

General information: TITO was launched using: RESULT: Template: 4WW7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1011 -49708 -49.17 -220.92 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -49.17 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.169

(partial model without unconserved sides chains): PDB file : Tito_4WW7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ww7-query.scw PDB file : Tito_Scwrl_4WW7.pdb: