Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRLLPNPERAWERAAHVQTLARAEIERRIPPFTGEVEVLSGGHANVNVRLGAERVLRVYRRDASAAPKEASL-----LAHGWRSFRVPAVLSAGDDFLVLEHVP--HVPLQDTAMCGAAVGRALAEIHGVGYDSAGFLGPELAVSVPFADCIGALRGHVDAVLDRLAPALRGE-----LSERVRAFLDANAEALRALAS-----------APVLLHGDFKASNLHWLSSGELLVLDW-EFAYAGPGLMDVGQLLRWSP---GREFVAAFAGAYRESAPLPEEFERWAAAFDLF-NLVGLLDGVEPGSRRAADVCGRISEVVER 3UZR Chain:A ((40-300)) ---------------------------------NELRYLSSGDDSDTFLCNEQYVVKVPKRDSVRISQKRELELYRFLENCKLSYQIPAVVYQSDRFNIMKYIKGERITYEQYHKLSEKEKDALA------YDEATFLKELHSIEI---DCSVSLFS--DALVNKKDKFLQDKKLLISILEKEQLLTDEMLEHIETIYENILSNAVLFKYTPCLVHNDFSANNMIFRNNRLFGVIDFGDFNVGDPD-NDFLCLLDCSTDDFGKEFGRKVLKYYQHKA--PEVAERKAELNDVYWSIDQIIYGYERKDR---------------

General information: TITO was launched using: RESULT: Template: 3UZR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 926 6448 6.96 27.67 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 6.96 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.238

(partial model without unconserved sides chains): PDB file : Tito_3UZR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3uzr-query.scw PDB file : Tito_Scwrl_3UZR.pdb: