Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSAPTEEMISVQKLVERLNAEYAF--EVDYIGADLGGFSSQNNQLTLKDKRRMVLKRCDQSQW--D-P---RNVDIATILAHHSSLIYAPLATVGGSSTFKFDGWVYAVFPYVDGEKLHQHSLDAETLKSAARALYAFHHIAHFAGSHAAQR--PNV---SDF-EREVTSVLEPTLSL--STDHADIIIRSIEAKRHILHSFSSDLSSINVGASSVIHGDFHNENILYRS--DHVVAILDFEMLHWGNVEDDIISFIALGCCNNGF-----S--EENLRMANIFVKEYLS--LHGENLDLHTALLRFIHLKSSSLFLQKMLIYTGNMLYAQIIERDNKLFPSIMKKHREISKTLSSL 4OCV Chain:A ((24-312)) ----------TNEALFDVASHFALEGTVDSIEPYGDGHINTTYLVTTDGP-RYILQRMNTGIFPDTVNLMRNVELVTSTLKAQGKETLDIVRTTSGDTWAEIDGGAWRVYKFIEHTMSYNLVPNPDVFREAGRAFGDFQNFLSG-FDANQLTETIAHFHDTPHRFEDFKKALA-ADELGRAAGCG-PEIEFYLSHADQYAVVMDGL-RDGSIPLRVTHNDTKLNNILMDATTGKARAIIDLDTIMPGSMLFDFGDSIRFGASTALEDERDLDKVHFSTELFRAYTEGFVGELRDSITAREAELL-----------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4OCV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1074 -11827 -11.01 -45.14 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -11.01 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.333

(partial model without unconserved sides chains): PDB file : Tito_4OCV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ocv-query.scw PDB file : Tito_Scwrl_4OCV.pdb: