Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGVKTQLSLHELNQLFPSYGFT---EIKPTISGIIDTTYIIHNE-TTGYILKRYERD--ITRKIELDIKLLNELKSVGLNVPACLD---------SNYGWYIYEKLEGKQPTNTKSYHIQALGRFLAKMHSQTSKMRCDSNRIIEDEV-TQS---LKYVKE---NFF----AYYKRFEFL----KNF--THKHEAIIHGDIFKDNTIFNGRKIGVIDFIDSSCGTFAYDVAVTLVGFDARERHDYYINLFLKNYNQHAPKKLSK---KVVKEKMKFAANFFALKRVHEYKNTSRAKELLK 1ZYL Chain:A ((8-281)) ---FQTLHPDTIMDALFEHGIRVDSGLTPL-NSYENRVYQFQDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFYFAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFI--HRPTIGLNEYLIEPRKLFEDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRD-GPMFVDLDDARNGPAVQDLWMLLNGDKAE--QRMQLETIIEAYEEFS--EFDTAEIGLIEPLRAMRLVYYLAWL---------------

General information: TITO was launched using: RESULT: Template: 1ZYL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 974 21679 22.26 90.71 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 22.26 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.426

(partial model without unconserved sides chains): PDB file : Tito_1ZYL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1zyl-query.scw PDB file : Tito_Scwrl_1ZYL.pdb: