Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLRRAELDDDLGRRRAATPAPQRVEELLAVELPAVGEELDGAVVPIDYGTSTELTVTAPDLFAALSRHPRAGRIVASVGDVRRLSGGFSKEMLMARATYSDGSGERFVVRKVAPGRRADGLRAEYDVLSSAFHSGCPVPEPWWYDSEALGTPAIATSALSGTPAGDPNGWVAPPSSAVLASVARAAAQQHQVELAAFDANPLPPLISAEDRREALSERRAVLDGLWAEGDDAWAPAFNLVLNWLESAIPEPADPPVLVHGDFGPHNFLIEGDELSGILDWERSHAGLAVEDLAYLRPTLDDE-SWTAFMREYVAAGGREPSDDALVWYIVWQDFWRAVSAYRMRSLFLQSPDQVLYGISGLLLAPRFLTSAVRSVSSAHAVAGARAC 1J7L Chain:A ((2-264)) ---------------AKMRISPELKKLIE----------KYRCVKDTEGMSP--------------------------AKVYKLVGENENLYLKMTDSRYKGT--------------TYDVEREKDMMLWL-EGKLPVPKVLHFERHD-GWSNLLMSEADGVLCSEEYE-DEQSPEKIIELYAECIRLFHSIDIS--DCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDTPFKDPRELYDFLKT--EKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF----------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1J7L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1257 -70094 -55.76 -267.53 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -55.76 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.240

(partial model without unconserved sides chains): PDB file : Tito_1J7L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1j7l-query.scw PDB file : Tito_Scwrl_1J7L.pdb: