Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGVKTQLSLHELNQLFPSYGFT---EIKPTISGIIDTTYIIHNE-TTGYILKRYERDI-TRKIELDIKLLNELKSV-GLNVPACLDSN-----YGW----YIYEKLEGKQPTN-----TKSYHIQALGRFLAKMHSQTSKMRCDSNRI---IEDEVTQSLKYVKENFF------AYYKRFEF-LKNF--THKHEAIIHGDIFKDNTIFNG-RK-IGVIDFIDSSCGTFAYDVAVTLVGFDARERHDYYINLFLKNYNQHAPKKLSK--KVVKEKMKFAANFFALKRVHEYKNTSRAKELLK 5IGI Chain:A ((2-284)) -TVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA-VIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVADDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVFG-----EEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFALDS------------

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1024 28468 27.80 115.72 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 27.80 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.406

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: