Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------MISNTPSFPASWNIEAAKRIADTPAGIVYELTRKDGSLAIAKVLKKKVLKDSLRGADFIAWRAG-------IGCVELLDQSDNILLMEHAGTETLRDVLFRDGND-----DATTEIAAEVLLRYHQPSEQSPPSSLLTLPLYFESLFRKAEQ----------------DRSDGVDSPFVEAARLAQTLIDQQRDIKP-----LHGDLHHENIMHSDRGWIIIDPA-GLIGDAALDVANMFSNPLDRFDLTRSEARIASMAAIFSRALQRDERLLLQYAFAYGCLSATWHEEDGNAEERDNELAVAATIKAVLKHF------------------------------------------------------ 2OLC Chain:A ((5-396)) KTPLYETLNESSAVALAVKLGLTLTCQEIGDGNLNYVFHIY--DRALIIKQAVPYWPLTIDRARIESSALIRQGEHVPHLVPRVFYSDTEMAVTVMEDLSHLKIARKGLIEGENYPHLSQHIGEFLGKTLFYSSDYALEPKVKKQLVKQFTNPELCDITERLVFTDPFFDHDTNDFEEELRPFVEKLWNNDSVKIEAAKLKKSFLTSAETLIHGDLHTGSIFASEHETKVIDPEFAFYGPIGFDVGQFIANLFLNALSRDGADREPLYEHVNQVWETFEETFSEAWQKDSLDVYANIDGYLTDTLSHIFEEAIGFAGCELIRRTIGLAHVADLDTIVPFDKRIGRKRLALETGTAFIEKRSEFKTITDVIELFKLLVK

General information: TITO was launched using: RESULT: Template: 2OLC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1301 13391 10.29 48.00 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 10.29 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.338

(partial model without unconserved sides chains): PDB file : Tito_2OLC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2olc-query.scw PDB file : Tito_Scwrl_2OLC.pdb: