Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGVKTQLSLHELNQLFPSYGFT---EIKPTISGIIDTTYIIHNE-TTGYILKRYERDI-TRKIELDIKLLNELKSV-GLNVPACLDSN-----YGW----YIYEKLEGKQPTN-----TKSYHIQALGRFLAKMHSQTSKMRCDSNRI---IEDEVTQSLKYVKENFF------AYYKRFEF-LKNF--THKHEAIIHGDIFKDNTIFNG-RK-IGVIDFIDSSCGTFAYDVAVTLVGFDARERHDYYINLFLKNYNQHAPKKLSK--KVVKEKMKFAANFFALKRVHEYKNTSRAKELLK 5IGH Chain:A ((1-284)) MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTAMVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVADDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVFG-----EEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFALDS------------

General information: TITO was launched using: RESULT: Template: 5IGH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 982 -147 -0.15 -0.60 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -0.15 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.215

(partial model without unconserved sides chains): PDB file : Tito_5IGH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igh-query.scw PDB file : Tito_Scwrl_5IGH.pdb: