Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIESQAPMLE-MLWEPHDPRHALAERFGFSDSE----SASRWVAAMLDEHWGIRIDSCERILMS-GGNALAWVGTP-SGRLLAK-----WSVVLDCFPRL-------METARLTNWLHGRGLPVSVPVPTR-DGCLQVEVDR-VSMGLQREIVGDL-LDTG---------DLNQVRAAGVILAQLQDALAAY---------PDADQFLAP-VVSSKPL---------------TVRVTDWLDSRADHLPMAA-RDTLRGLVASAPP----DRLPRQLVHF-------DIRSANILWACGGVAAILDFEEAQHDHRIVELARAAVLLGTRY---HNWGPVSADVRTEFLTGYQSERLLTPAEAGWLHIVLLWQALAMVPPGDDPTGWGPSALSQLSQETAR-------------------- 5L7J Chain:A ((7-459)) TISGVTPVAVMTKPLPCPGKCIYCPTFAATPQSYTPESPAVLRAKSCEYQAYKQVALRLRIIQDMGHPTDKVELIIMGGTFLSADITYQYGFIKDCYDALNGVVAGSLEEAKTINE-TAQHRCVGLCIETRPDICGKAEIQRMIDFGTTRVELGVQMLDDDIYKLVERGHRVSDVAEATCLLREYGLKVHYHWMPGLPGSSPEKDLALSRMVFEDPRFCPDGLKLYPTMVVEGTILEQWWKEGRYTPYPNGTMTGLIADIKALVPPYVRISRVKCRCIRCREYSGEPTLRRLDYPAS-GGKEIFLSFEDASDT--LYGLLRLRIPCASLPVLGQKYGAKTGLVRE--LHVYGTELSLGH---RGLGRKLLAEAECLARDE-----FGLDSLAILSGVGAREYYRSLGYELVAGYMCKHLD

General information: TITO was launched using: RESULT: Template: 5L7J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1576 14278 9.06 45.33 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 9.06 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.201

(partial model without unconserved sides chains): PDB file : Tito_5L7J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5l7j-query.scw PDB file : Tito_Scwrl_5L7J.pdb: