Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTPGDRMMASALGSWTRLAPFAGLDPAAFEAEELWRREDPGQVRIVLRMRGGAGQDLVFKRVFQPQD--RGRWAEALDGQRRAVRALMRDPVHVPPAILAED------------AETLSTVQDFVPGRTLQDHMLLRFDKPGARAQVLQRAGRFLAAFHGATAEGDKPF--NPATM-LDYIRALGRRA---LSG-DLTLADPDLFAVLAEGLEAAAQTAAGQPVRVAAQHGDLHARNLIVSG----KRATLIDFAQDRSAPVQYDMARLIVDLGARFSGDAAPEATCGLPAADLAALSDGYGRDLSQDACLAFLCRCRVLQDWAALPAVASQRSAFQQERLLQLQKTATRLAAA 6EF6 Chain:A ((27-297)) ----------DVTIAQQALTHYDVS-DNASLRLL-----NLSENATYLVEDG-EHQSILR-VHRQDYHQPHEIESELDWLAALRT----DSDVTVPTVVPARDGRRVVTVDPADVPRHVVHFEMVGGAEPDEES--------LTLDDFQTLGRITASLHEHSQRWTRPAGFGRFSWDWEHCLGDTPRWGRWQDAEGVGASETALLTRAQDLLHRKLEEYGSGPDRYGLIHADLRLANLLVDSSTPQRTITVIDFDDCGFGWYFYDFGTAVSFIEHDPR-----------LGEWQESWVAGYRSRRELPA---------------------------------------------

General information: TITO was launched using: RESULT: Template: 6EF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1082 -7499 -6.93 -30.86 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -6.93 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.193

(partial model without unconserved sides chains): PDB file : Tito_6EF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6ef6-query.scw PDB file : Tito_Scwrl_6EF6.pdb: