Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSARIAERAMAALGRSGMGARATRVGGGRASEVFLVQGGDSELIAYWLPEGGAREARRRFALLERMAGQFRLAPRPLAVDEDGDAALLLVEKLEGVAPAACGPLAPDTVRRLAENFIGTLASLHALE-----VAPADRPPDYLRRILSEWRRRWNAEEAGGAIDADFSAVADWLTERMPETAPA-----AWLHNDYKLDNILVDPGDPARVVGVVDWELAAVGHPLADLGAALAYWIESRDSSLLRLDAPGPSCAPGAPTREALVDLYAAAAGREVTEPAYWYAYGLLRLAVITQQLALRRRDEGQRVPRAKLIVRWLLDRAAEACGGGRL 3HAM Chain:A ((1-299)) -MVNLDAEIYEHLNKQIKINELRYLSSGDDSDTFLC--NEQYVVKVPKRDSVRISQKREFELYRFLENCKLSYQIPAVVYQSDRFNIMKYIKGERITYEQYHKLSEKEKDALAYDEATFLKELHSIEIDCSVSLFSDALVNKKDKFLQDKKLLISILEKEQLLTDEMLEHIETIYENILNNAVLFKYTPCLVHNDFSANNMIFRNN---RLFGVIDFGDFNVGDPDNDFLCLLDCS--------------------TDDFGKEFGRKVLKYYQHKAPEVAERKAELNDVYWSIDQIIYGYERKDREMLIKGVSELLQTQAEMFIF------

General information: TITO was launched using: RESULT: Template: 3HAM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1270 -30670 -24.15 -106.12 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -24.15 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.367

(partial model without unconserved sides chains): PDB file : Tito_3HAM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ham-query.scw PDB file : Tito_Scwrl_3HAM.pdb: