Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFDNPDRSQNWSKTGKIADSSDLLPRFPELQRQPCQITSLGSAGGLSGGAIWRIECDRRLYALRRWPAETTSARLRYVHSLQRLWRESNLKFIPELYTTAEGESFTENALGFWELAEWMPGAALEGDTISPQQRDAVAAAIAAIHQAA-AQHESSLEPSPGLRQRLTMLQRWRTMDRT----MLQ--D-----AIQRLGWPEFAAIAQQMLQSFAYAANTIGD-EL-QAIVSTPLPLHACLRDIHREHIFLTGALVSGVLDFGAVRIESRAGDLARLCGSLFEDD---R-NRWDDFLARYERLRPLSAAERRAIAVFDRSAVLLTGLQWIEWIALERRQFPDPAAVLSRLDISLRRLQFLLK 2Q83 Chain:A ((22-307)) ------------------LAENVLQGWDV---Q--AEKIDVIQ----A-LVWKVHTDSGAVCLKRIHRP--EKKALFSIFAQDYLAKKGMN-VPGILPNKKGSLYSKHGSFLFVVYDWIEGRPFEL--TVKQDLEFIMKGLADFHTASVGYQPPNGVPIFT------KLGRWPNHYTKRCKQMETWKLMAEAEKEDPFSQ-LYLQEIDGFIEDGLRIKDRLLQSTYVPWTEQLKKSPNLCHQDYGTGNTLLGENEQIWVIDLDTVSFDLPIRDLRKMIIPLLDTTGVWDDETFNVMLNAYESRAPLTEEQKQVMFIDMLFPY----------------------------------------

General information: TITO was launched using: RESULT: Template: 2Q83.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1052 -35248 -33.51 -133.51 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -33.51 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.299

(partial model without unconserved sides chains): PDB file : Tito_2Q83.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2q83-query.scw PDB file : Tito_Scwrl_2Q83.pdb: