Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEAARETRLPCAAMAILTPLSLADARRIGALYGLEIADVRGLLAGSVNSNFALTLADGRGQVFLRVYEEQQL--GAASREARMLEHLAAGGVATPQPLRRRDAAPSDTDAASDGAAFVAEHAGKPVALFPWVGGASLCQARVTPDATRRVGAALARVHLVGASFEGANASRFDLDGLDQRLRGLRSPAGARSTAPAPALPPDVAAAVDELTGRLERIRAAAPRTPGPQTLIHGDLFRDNVLWEAGEISALLDFESASRGSAAFDLAVTMLAWCFGDDLDPDLVSALAAGYTAVRPLSTEERD--RLFHESVIAALRFSITRITDFELRPKGSGVYKDFRRFLARQRTLERLGPEGLLALLGV---- 1ZYL Chain:A ((4-328)) SAFTFQTLHPDTIMDALFEHGIRVDSGLTPLNSYE------------NRVYQFQDEDRR-RFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQT-------------LLNHQGFYFAVFPSVGGRQF--EADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLFEDATL--------IPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDG--PMFVDLDDARNGPAVQDLWMLLNGD---KAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVYYLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY

General information: TITO was launched using: RESULT: Template: 1ZYL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1482 -5665 -3.82 -17.87 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -3.82 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.331

(partial model without unconserved sides chains): PDB file : Tito_1ZYL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1zyl-query.scw PDB file : Tito_Scwrl_1ZYL.pdb: