Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MISNTP-SFPASWNIEAAKRIADTPAGIVYELTRKDG-----------------SL-AIAKVLK-----KKVLKDS-LRGADFIA------------WRAGIGCVELLDQSDNIL-LMEHAGT-ETLRDVLFRDGNDDATTEIAAEVLLRYHQP---------------SEQSPPSSLL-TLPL--YFES-L-FRKAEQDRSDGVDSPFVEAARLAQTLIDQQRDIKPLHGDLHHEN-----IMHSDRGWIIIDPAGLIGD------------AALDVANMFSNPL-------DRFDLTRSEAR---------------IASMAAIFSRALQRDERLLL-------------------------------QY-AFAYGCLSA-TWHEEDGNAEERD-NELAVAATIKAV-LKHF 4QT9 Chain:A ((19-432)) LSDDALMDTVQRRTFLYFWEGAEPNSGLAPERYHVDGVYPQNDANVVTSGGSGFGIMAILAGIDRGYVTREEGLARMERIVSFLEKADRFHGAYPHWWYGDTGKVKPFGQKDNGGDLVETAFLMQGLLAVHQYYANGNDKEKAIAQRIDRLWREVDWDWYRKGGQNVLYWHWSPTYGWEMDFPIHGYNECMIMYILAAASPTHGVPAAVYHDGWAQNGAIVSPHKVEGIELHLRYQGTEAGPLFWAQYSFLGLDPVGLKDEYCPSYFHEMRNLTLVNRAYCIRNPKHYKGFGPDCWGLTASYSVDGYAAHSPNEQDDKGVISPTAALSSIVYTPEYSLQVMRHLYNMGDKVFGPFGFYDAFSETDNWYPQRYLAIDQGPIAVMIENYRTGLLWKLFMSHPDVQAGLTKLGFNTN

General information: TITO was launched using: RESULT: Template: 4QT9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1388 2097 1.51 7.46 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 1.51 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.267

(partial model without unconserved sides chains): PDB file : Tito_4QT9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4qt9-query.scw PDB file : Tito_Scwrl_4QT9.pdb: