Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSAPTEEMISVQKLVERLNAEYAF-------EVDYIGADLGGFSSQNNQLTLKD--------KRRMVLKRCDQSQWDP--RNVDIATILAHHSSLIYAPLATVGGSSTFKFDGWVYAVFPYVDGEKLHQHSL-DAETLKSAARALYAFHHIAHFAGSHAA-QRPNVSDF-EREVTSVLEPTLSL-STDHADIIIRSIEAKRHILHSFSSDLSSINVGASSVIHGDFHNENILYRSD------HVVAILDFEMLHWGNVEDDIISFIALGCCNNG----------F-SEENLRMANIFVKEYLSLHGENLDLHTALLRFIHLKSSSLFLQKMLIYTGNMLYAQIIERDNKLFPSIMKKHREISKTLSSL 5FTG Chain:A ((9-309)) ----------RRRAYLWCKEFLPGAWRGLREDEFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG----EAMVLESVMFAILAERSLG-PKLYGIFPQ----------GRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMP-FNKEPKWLFGTMEKYLKEVLRIKF-TEESRIKKLHKLLSYNLPLELENLRSLLE----STPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQN-------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5FTG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 890 3918 4.40 16.46 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : 4.40 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.200

(partial model without unconserved sides chains): PDB file : Tito_5FTG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ftg-query.scw PDB file : Tito_Scwrl_5FTG.pdb: